JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814867.1
Database:	nt	Go to original record
Description:	Rotheca serrata chloroplast, complete genome.
Taxon:	Rotheca serrata
Original size:	150371 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	114618	115169	-	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaagcaa aggttcaaaa
151   aaagaaaaaa aaacaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ttggtagaag aatgtttcca tgggaaacat
251   gaaggataaa ggtatctatt gaatactata tcaaatgatt aatgatggcc
301   tgaatctttc tttttgaata tgaaaaatgc aagaattggt gtgaatggat
351   tccacattga agaaaaaatc gaatattcat tcatcaaatc attcactcca
401   tagtacgata gatcttttaa agatctcttt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aatactggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details