JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814860.1
Database:	nt	Go to original record
Description:	Caryopteris trichosphaera chloroplast, complete genome.
Taxon:	Caryopteris trichosphaera
Original size:	151380 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	115780	116335	-	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaccaa aggttcaaaa
151   aacgaaaaga aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgt gctggtagag gaatctttcc atggaaactt
251   cagaaaggat gaaagataaa tgtatctatt gaatactata tcaaatgatt
301   aatgatggcc cgaatctttt aatatgaaaa attgaagaat tggtgtgaat
351   tgattccaca ttgaagaaaa aatcgaatat tcattcatga aatcattcac
401   tccatagtct gatcgatctt ttcaagaact gattaatcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaataac ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details