JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814859.1
Database:	nt	Go to original record
Description:	Caryopteris mongholica chloroplast, complete genome.
Taxon:	Caryopteris mongholica
Original size:	151635 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	116051	116611	-	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaccaa aggttcaaaa
151   aacgaaaaga aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgtg ctggtagagg aatctttcca tggaaacttc
251   agaaaggatg aaagataaat gtatctattg aatactatat caaatgatta
301   atgatggccc gaatctgtat tttttaatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaaaaatcg aatattcatt catcaaatca
401   ttcactccat agtctgatcg atcttttcaa gaactgatta atcggacgag
451   aataaagata gagtcccgtt ctagatgtca ataacggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details