JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814858.1
Database:	nt	Go to original record
Description:	Amethystea caerulea chloroplast, complete genome.
Taxon:	Amethystea caerulea
Original size:	151890 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	116127	116685	-	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaccaa aggttcaaaa
151   aacgaaaaga aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgct ggtagaggaa tctttccatg gaaacttcag
251   aaaggatgaa ggataaatgg atctattgaa tactatatcg aatgattaat
301   gatggcccga atctgtattt tttaatatga aaaatggaag aattggtgtg
351   aattgattcc acattgaaga aaaaatcgaa tattcattca tcaaatcatt
401   cactccatag tccgatcgat cttttcaaga actgattaat cggacgagaa
451   taaagataga gtcccattct acatgtcaat accggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details