JRC GMO-Amplicons | EURL GMFF

Record ID:	MN814852.1
Database:	nt	Go to original record
Description:	Ajuga campylanthoides chloroplast, complete genome.
Taxon:	Ajuga campylanthoides
Original size:	150500 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	115580	116126	-	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaacccagg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttca ctgcggaatc tttccatgga aacttcagaa aggatgaagg
251   ataaatggat ctattgaata ctatatcgaa tgattcacga tggcccgaat
301   ctgtattttt taatatgaaa aatggaagaa ttggtgtgaa ttgattccac
351   attgaagaaa aaatcgaata ttcattcatc aaatcattca ctccatagtc
401   cgatagatct tttcaagaac tgattaatcg gacgagaata aagatagagt
451   cccgttctac atgtcaatac cggcaacaat gaaatttata gtaataggaa
501   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details