JRC GMO-Amplicons | EURL GMFF

Record ID:	MH464896.1
Database:	nt	Go to original record
Description:	Dictyanthus macvaughianus voucher E.W. Molseed 220 (MEXU) tRNA-Leu (trnL) gene and trnL-F intergenic spacer, partial sequence; chloroplast.
Taxon:	Dictyanthus macvaughianus
Original size:	945 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	553	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   atgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag ttggtagaga aatctttcca tcaaaaatta
251   agaaaggatg aaagatatac atacgtattg aatactagcg gacccaaatg
301   agtctatatt ttttctataa aaaacgtaag aattggtgtg atttgattct
351   ttcttcaatg tggaatcgaa tattcattga tcaaaagatt cactccatag
401   tctgtagatc ctcttttcaa gaaccggata aatcggacga gaataaagat
451   agagtcccgt tctacatgtc aatgctggca acaatgaaat ttttagtaag
501   aggaaaatcc gtcgacttaa aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details