JRC GMO-Amplicons | EURL GMFF

Record ID:	MN687904.1
Database:	nt	Go to original record
Description:	Ocimum tenuiflorum chloroplast, complete genome.
Taxon:	Ocimum tenuiflorum
Original size:	152419 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46950	47497	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aagttcaaaa
151   aacgaaaaaa aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatggg agttgactgc gctggtagag gaatctttcc atggaaactt
251   tcgaaaggat gaaggataaa cgcatctatt gaatactata tcaaatggtt
301   aatgatggcc caaatctttt aatatgaaaa aatggaaaaa ttggtgtgaa
351   ttgattctac gttgaagaaa aaatcaaata ttcatcaact cattcgctcc
401   atagtccgat agataaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccattcta catgtcaata tcggcaacaa tgaaatttat agtaagagga
501   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details