JRC GMO-Amplicons | EURL GMFF

Record ID:	MK944405.1
Database:	nt	Go to original record
Description:	Salvia yunnanensis chloroplast, complete genome.
Taxon:	Salvia yunnanensis
Original size:	151396 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46772	47292	+	521 bp

Amplicon:

521 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ttcaaaaaaa aggataggtg
151   cagagactca atggaagttg ttctaacaaa tggagttgac tgcgccggta
201   gaggaatctt tccacggaaa ctttagaaag ataaacccat ctattgaata
251   ctatatcaaa tgattaatgt tggcccgaat ctgttttttt aatatgaaaa
301   tggaaaaatc gatgtgaatt tctttcacgt tgaagaataa atcgaatatt
351   catcaactca ttcactccat agttcgatag atcttttaaa gaacttatta
401   atcggacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
451   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
501   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.34e-08
primerB	100.0	20 bp	0	0	521	502	2.34e-08

Match details