JRC GMO-Amplicons | EURL GMFF

Record ID:	MN514858.1
Database:	nt	Go to original record
Description:	Rheum acuminatum chloroplast, complete genome.
Taxon:	Rheum acuminatum
Original size:	161655 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49773	50382	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta ttataaaaat tattccatcg aaactccaaa aaagatgaag
251   aataaaccta tacgcagacg tacttaaata taaatactat atataataac
301   tatatatata tatgatataa aaaaaatgaa attaaaaaat attgatgacg
351   acccgcattt tttttatgac tatgaaaaaa tggaagaatt gttgtgaagc
401   gattccaagt tgaagaaaga gtcgaatatg cgtttaataa attaaagcat
451   ttattccccg gtctgagaga tcttttgaag aaccgatcca tcggatgaga
501   atgaagatag agtcccattc tacgtgtcaa tcctgacaac aatgaaattt
551   atagtaagag gaaaatccgt cgactttata aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details