JRC GMO-Amplicons | EURL GMFF

Record ID:	MT211650.1
Database:	nt	Go to original record
Description:	Trichosanthes rosthornii voucher KIOM201701018518 chloroplast, complete genome.
Taxon:	Trichosanthes rosthornii
Original size:	157097 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49541	50130	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcgttag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaacccg aatctctatt
301   tttttttttt tttttatatt tatataaaaa atgatatatg aaaatgaacg
351   aattgttctg aatcgattcc aatatctcca agttgaaaaa agaatcgaat
401   attcattgat caaatcattt actccatcat acatagtctg atagatcttt
451   tgaagaactg attaatcaga tgcgaataga ataaagatag agtcccattc
501   tacatgtcaa taccgacaaa aatgaaattt atagtaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details