JRC GMO-Amplicons | EURL GMFF

Record ID:	MT211649.1
Database:	nt	Go to original record
Description:	Trichosanthes kirilowii var. japonica voucher 2-17-0166 chloroplast, complete genome.
Taxon:	Trichosanthes kirilowii var. japonica
Original size:	157066 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49467	50052	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa gggtaggtgc
151   agagactcaa tggaagctgt tctaacaaat ggagttgact acgttgcgtt
201   agtaaaggaa tgaatccttc tatcgaaact ccagaaaaga tgaaagataa
251   acgtattacg tactgaaata ctatttgatt aatgacaacc cgaatctcta
301   tttttttttt tttatttata taaaaaatga tatataaaaa tgaacgaatt
351   gttctgaatc gattccaata tctccaagtt gaaaaaagaa tcgaatattc
401   attgatcaaa tcatttactc catcatacat agtctgatag atcttttgaa
451   gaactgatta atcagatgcg aatagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaaaaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details