JRC GMO-Amplicons | EURL GMFF

Record ID:	MT211648.1
Database:	nt	Go to original record
Description:	Trichosanthes kirilowii var. japonica voucher KIOM201701018518 chloroplast, complete genome.
Taxon:	Trichosanthes kirilowii var. japonica
Original size:	157155 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49520	50106	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa agggtaggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tacgttgcgt
201   tagtaaagga atgaatcctt ctatcgaaac tccagaaaag atgaaagata
251   aacgtattac gtactgaaat actatttgat taatgacaac ccgaatctct
301   attttttttt ttttatttat ataaaaaatg atatataaaa atgaacgaat
351   tgttctgaat cgattccaat atctccaagt tgaaaaaaga atcgaatatt
401   cattgatcaa atcatttact ccatcataca tagtctgata gatcttttga
451   agaactgatt aatcagatgc gaatagaata aagatagagt cccattctac
501   atgtcaatac cgacaaaaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details