JRC GMO-Amplicons | EURL GMFF

Record ID:	MN508249.2
Database:	nt	Go to original record
Description:	Physalis chenopodifolia chloroplast, complete genome.
Taxon:	Physalis chenopodiifolia
Original size:	156900 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49219	49801	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatattgaa
301   tactatatca aatgattaat gacgacccga atccgtattt tttctataaa
351   aaatagaaga attggtgtga atcgattcta cattgaagaa agaatcgaat
401   attcattgat caaatcattc aattcactcc atagtctgat agatcttttg
451   aagaactgat taatcggacg agaataaaga tagagtcccg ttctacatgt
501   caatgccggc aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details