JRC GMO-Amplicons | EURL GMFF

Record ID:	KY378687.1
Database:	nt	Go to original record
Description:	Anthospermum spathulatum chloroplast, partial genome.
Taxon:	Anthospermum spathulatum
Original size:	127269 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47659	48248	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctgag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctatttt ccgaaaacaa aggttcagaa
151   agtgaaaaaa gggaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg gtagtcaaga agtctttcca tcaaaaattc
251   ataaactata agtattaagg atgaagtgaa ggataaacgt atatacatac
301   gtagtcaata gtatattaaa tgagtaatga taacccgaat gaatctttat
351   ttttctataa aaaaataaag aattgttctt aatagattcc acgtttagaa
401   aagaatcgaa tattcattga tcaaatgatt cactccatag tctgatagat
451   cctttcacga actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat gtcggcaaca atgaaattta tagttaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details