JRC GMO-Amplicons | EURL GMFF

Record ID:	KY378684.1
Database:	nt	Go to original record
Description:	Kohautia caespitosa chloroplast, partial genome.
Taxon:	Kohautia caespitosa
Original size:	128658 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47641	48246	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gattgggcct tggtatggaa
51    acccgctaag tgagaacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggtaat cctgagccaa atcctatttt ccgaaactaa aaaacaaaga
151   ttcagaaagt gaaaaggggg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttggatgc gttagtcgag aagtccttcc agtaaaaatt
251   cataaagtat aagtgtttaa ggatgaagtg acggataaac atatatacat
301   atatatattg aatagtatat tcaataaatg agtaatgaca gccccaaacg
351   aatctctatt ttttctatga aaaaataaag aatggttgtt aatagattcc
401   acgtttaagt ttaagaaaga atcgaatatt cattgattaa atgattcact
451   ccatagcctg atagatcttt tcaccaactg attaatcaga cgagaataaa
501   gatagagtcc cattttacat gtcaatgccg gcaacaatga aatttattgt
551   taagaggaaa atccgtcgac ttttaaaatc gtgagggttc aagtccctct
601   atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details