JRC GMO-Amplicons | EURL GMFF

Record ID:	KT279373.1
Database:	nt	Go to original record
Description:	Bothriochloa ewartiana isolate 723 tRNA-Thr gene, partial sequence; trnT-trnL intergenic spacer and tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Bothriochloa ewartiana
Original size:	1604 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	747	1272	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggga
51    aacctgctaa gtggtaactt ccaaattcag agaaaccctg gaatgaaaaa
101   tgggcaatcc tgagccaaat cccttttttg aaaaaacaag cggttctcaa
151   actagaaccc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaagtaataa cgattaatca cagaacccat attataatat
251   aggttcttta ttttattttt agaatgaaat taggaatgat tatgaaatag
301   aaaattcata attttttgag aattattgtg aatctattcc aatcgaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatttt ttaaaaagtg
401   gattaatcgg acgaggataa agagagagtc ccattctaca tgtcaatact
451   gacaacaatg aaatttctag taaaaggaaa atccgtcgac tttataagtc
501   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details