JRC GMO-Amplicons | EURL GMFF

Record ID:	MK165240.1
Database:	nt	Go to original record
Description:	Atractocarpus longistipitatus voucher Mouly 742 (P) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Atractocarpus longistipitatus
Original size:	1663 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	833	1388	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagccc tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct acgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcgactga atctatgaaa acggaagaat tggtgtgaat
351   agattccaca ttgaagaaag aatcgaatat tcattgatca aatgattcac
401   tccatagtct gatagatctt ttcaagaatt gattaatcgg gcgagaataa
451   agatagagtc ccgttctaca tgtcaatgtc ggcaacaatg aaatttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details