JRC GMO-Amplicons | EURL GMFF

Record ID:	MN509267.1
Database:	nt	Go to original record
Description:	Lilium speciosum var. gloriosoides chloroplast, complete genome.
Taxon:	Lilium speciosum var. gloriosoides
Original size:	152912 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45447	46060	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttgattttg agaaaaaggg tttaatttat
151   agtttttata atataaaact acaataaaaa aagataggtg cagagactca
201   atggaagctg ttctaacgaa tgaagttgat tacgttgcac tggtagccgg
251   aatccttcta tcaaaattac ggagaggatg cccgccctat atacgtatat
301   atacatactg acatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatgaaaaa tttaagagtt attgtaaatc tattccatat
401   tccaatcaaa gtttaaggaa gaatcgaata ttcagtgatc aaattattct
451   tattcattcc agagtctgta tagatctttt taaaaactga ttattcggac
501   gagaataaag agagagtccc attctacgtg tcaataccga caacaatgaa
551   atttatagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details