JRC GMO-Amplicons | EURL GMFF

Record ID:	MN115429.1
Database:	nt	Go to original record
Description:	Ladeania lanceolata voucher Ahrendsen 24 chloroplast, partial genome.
Taxon:	Ladeania lanceolata
Original size:	126014 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13302	13882	-	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccctgg aattcacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga aaagttcaga
151   aagtgataat aaaaaaggga taggtgcaga gactcaatgg aagctattct
201   aacaaatgga gttgacgatt tttccttttt gcattaggaa aagaatcctt
251   ccattaaaat tccaggaatg gatcaaagag aaacatatat atatactgaa
301   atactatttc aattgattaa tgacgatcca tttgtgataa aaatattcac
351   aaatgaaaga tgtgaatcaa atcaattcca agttgaagaa aagatggaat
401   attcattgat caaattattc attccatcat aatctgatag atcccttgaa
451   gaactgatca atcagacgag aataaagata gagtcctatt ctacatgtca
501   atactgacaa caatgaaatt tatagtaaga ggaaaatccg tcgatttaat
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details