JRC GMO-Amplicons | EURL GMFF

Record ID:	MN115424.1
Database:	nt	Go to original record
Description:	Pediomelum digitatum voucher Jones 1022 chloroplast, partial genome.
Taxon:	Pediomelum digitatum
Original size:	126051 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	13290	13874	-	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct tggtatggaa
51    acttaccaag tgagaacttt caaattcaga gaaaccctgg aattcacaac
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga aaagttaaga
151   aagtgataat aaaaaaggga taggtgcaga gactcaatgg aagctattct
201   aacaaatgga gttgacgatt tttccttttt gtattaggaa aagaatcctt
251   ccataaaaat tccaggaatg gatcaaagag aaacatatat atactgaaat
301   actatttcaa ttgattaatg aagattcatt tgtaataaaa atattcacaa
351   atgcaaatga aagatgtgaa tcaaatcaat tccaagttga agaaacgatg
401   gaatattcat tgatcaaatt attcacttca tcataatctg atagatccct
451   tgaagaactg atcaatcaga cgagaataaa gatagagtcc tattctacat
501   gtcaatactg acaacaatga aatttatagt aagaggaaaa tccgtcgatt
551   taataaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details