JRC GMO-Amplicons | EURL GMFF

Record ID:	MH935322.1
Database:	nt	Go to original record
Description:	Cnidoscolus elasticus voucher C.A. Maya-L. & al. 618 (IEB) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cnidoscolus elasticus
Original size:	1044 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	602	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcca aaaacaaaca aagtaaagac
151   agaataaaat aaaaaaaaaa gggggggata ggtgcagaga ctcaacggaa
201   gctgttctaa taaatggagt tgactacgtt gcattagtaa agtaaagaaa
251   tccttccgtc aaaacttcag aaaggataac cgtatataca tacgtatacg
301   tactgaaata ctatctcaaa tgattaagat taataggggc ccaaatccgt
351   atttttttat ttcatttttc atataaagat gaaataaaaa aaattgtttt
401   gaattgattc caagttgaag aaagggttga atattaattg atcaaatcat
451   tcattccaaa gtctgataga taactgatta atcggacgag aataaagata
501   gagtcccatt ctacatgtca atatcgacaa caaggaaatt tatagtaaga
551   ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details