JRC GMO-Amplicons | EURL GMFF

Record ID:	MH935306.1
Database:	nt	Go to original record
Description:	Cnidoscolus populifolius voucher C.H. de M. Fernandes 7 (MO) tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cnidoscolus populifolius
Original size:	1035 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	599	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaca aagtaaagac
151   agaataaaat aaaaaaaagg gggggatagg tgcagagact caacggaagc
201   tgttctaata aatggagttg actacgttgc attagtaaag taaagaaatc
251   cttccgtcaa aacttcagaa aggataaccg tatatacata cgtatacgta
301   ctgaaatact atctcaaatg attaagatta ataggggccc aaatccgtat
351   ttttttattt catttttcat ataaagatga aataaaaaaa attgttttga
401   attgattcca agttgaagaa agggttgaat attaattgat caaatcattc
451   attccaaagt ctgatagata actgattaat cggacgagaa taaagataga
501   gtcccattct acatgtcaat atcgacaaca aggaaattta tagtaagagg
551   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details