JRC GMO-Amplicons | EURL GMFF

Record ID:	MH884563.1
Database:	nt	Go to original record
Description:	Plectranthus hadiensis isolate P.had.1 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Coleus hadiensis
Original size:	1625 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	729	1279	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agattcacaa
151   aacgaaaagg ataggtgcag agactcaatg gaagctgttc taacaaatgg
201   gagttgactg cgctggtaga ggaatctttc catggaaact ttagaaagga
251   tgaaggataa acgcatctat tgaatactat atcaaatggt taatgatggc
301   ccgaaccttt taatatgaaa aaatggaaaa attggtgtga attgattcta
351   cgttgaagaa aaaatcgaat attcatcaac tcattcgctc catagtccga
401   tagatctttt aaagaactga ttaatcggac gagaataaag atagagtccc
451   attctacatg ctacatgtca atatcggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details