JRC GMO-Amplicons | EURL GMFF

Record ID:	MN268502.1
Database:	nt	Go to original record
Description:	Eragrostis pilosa chloroplast, complete genome.
Taxon:	Eragrostis pilosa
Original size:	134815 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46812	47416	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aatgaaaagg ataggtgcag agactcaatg gaagctattc
201   taacgaatcg aggtgtaatt aaatccttca attcattgtt tttgagcaaa
251   gaagggcttt atacatctaa tacacacgta tagatactga catagcaaac
301   gattaatcac agaacccata tcaatcctga gccaaatccc ttttttgaaa
351   aaacaagtgg ttctcaaact agaacccaaa tgaaaaggat aggtgcagag
401   actcaatgga agctattcta acgaatcgag gtgtaattaa atccttcaat
451   tcattgtttt tgagatcttt taaaaagagg attaatcgga cgaggataaa
501   gagagagtcc cattctacat gtcaatactg acaacaatga aatttctagt
551   aaaaggaaaa tccgtcgact ttataagtcg tgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details