JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ492301.1
Database:	nt	Go to original record
Description:	Ramonda myconi chloroplast tRNA-Leu gene (partial), trnL-F intergenic spacer and tRNA-Phe gene (partial)
Taxon:	Ramonda myconi
Original size:	958 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	556	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatcga aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ctattgatga ttaataatgg cccgaatctg
301   tatctgattt tttgtatatg aaaaatggaa gaattggtgt gaattgattt
351   cacattgata aaagaatcga atattcattg atcaaatcat tcactccata
401   gtccgataga tctcttaaag aacttattat tattattcgg acgagaataa
451   agatagagtc ccgttctaca tgtcaatacc ggcaacaatg aaacttatag
501   taagaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details