JRC GMO-Amplicons | EURL GMFF

Record ID:	MN047312.1
Database:	nt	Go to original record
Description:	Scutellaria indica var. coccinea chloroplast, complete genome.
Taxon:	Scutellaria indica var. coccinea
Original size:	151956 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47655	48205	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggtga tcctgatcca aatcctgttt tctgaaaaca aaggctcaaa
151   aaacgaaaaa tgggataggt gcagagactc gatggaagct gttctaacaa
201   atggagttag ctgcgttgtt agaggaatcc tttccgcgga aacttcagaa
251   aggataaacg tatctattga atactatatc aaatgattaa tgatggcccg
301   aatctctatt tttgaatatg aaaaacggaa gaatcggtgt gaattgattc
351   cacattgaaa aaaaaaatgg aatattcatt catcaaatca ttcactccac
401   ggtccaatag gtcttttaaa gaactaatta atcggacgag aataaagata
451   gagtcccatt ctacatgtca ataccggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details