Match details
Record ID: |
M74305.1 |
Database: |
nt |
Go to original record |
Description: |
Synthetic expression vector pUC18cpPRV, constructed from cauliflower mosaic virus 35S gene-regulatory elements, papaya ringspot virus coat protein coding sequence, and the 5' untranslated region of cucumber mosaic virus coat protein, 5' end. |
Taxon: |
synthetic construct |
Original size: |
480 bp |
View other 6 matches for this record
View other 10904 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
206 |
284 |
+ |
79 bp |
|
1 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat
51 gacgcacaat cccactatcc ttcgcaaga
View other 6569 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
3.41e-10 |
primerB |
100.0 |
22 bp |
0 |
0 |
79 |
58 |
3.41e-10 |
probe |
100.0 |
26 bp |
0 |
0 |
31 |
56 |
2.52e-12 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.