JRC GMO-Amplicons | EURL GMFF

Record ID:	MN807280.1
Database:	nt	Go to original record
Description:	Cucumis melo subsp. melo chloroplast, complete genome.
Taxon:	Cucumis melo subsp. melo
Original size:	156577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49871	50472	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa aaaaagggta
151   ggtgcagaga ctcaatggaa gctgttctaa caaatggagt tgacgacgtt
201   gcgttagtaa aggaatgaat ccttccatcg aaactccaga aacgatgaaa
251   gataaacgta ttacgtactg aaatactata ttgattaatg acaactcgaa
301   tctctatttt ttatatttat atagatattt atatagataa aatatatata
351   taaaaatgaa cgaattgtta tgaatcgatt ccaagatctc caagttgaaa
401   aaagaatcga atattcattg atcaaatcat ttactccatc atacatagtc
451   tgatagatct ttttgaagaa ctgattaatc agatcgaata gaataaagat
501   agagtcccat tctacatgtc aataccgaca aaaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details