JRC GMO-Amplicons | EURL GMFF

Record ID:	KY860484.1
Database:	nt	Go to original record
Description:	Colchicum triphyllum voucher E. Kaya C1802 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Colchicum triphyllum
Original size:	1022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	616	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgatta gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc tttatgttaa gaaaacaaag gataggtgca
151   gagactcaat ggaagctgtt ctaacaaatg gaattgacgt tgactacgtt
201   gcgttggtag ctggaatccc tctatcgaaa ttacagaaag gatgacccta
251   tatgcgtata tatacatact gacatatcaa acgattaatc acaactcgaa
301   tctggaatcc atattattaa ctataatata tattatagtt attattatag
351   atatattttt atataaattt atagattaat taagagttat tataaatata
401   ttccagtcga agttgaagga agaatcgaat attcagtatt cagtgatcaa
451   atcattcatt ccggagtcgg atagatcttt tgaaaaaaat gattaatcgg
501   acgagaataa agagagagtc ctgttctacg tgtcaatacc gacagcaatg
551   aaatttatag tattaggaaa atccgtcgac tttagaaatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details