JRC GMO-Amplicons | EURL GMFF

Record ID:	MN223722.1
Database:	nt	Go to original record
Description:	Utricularia amethystina voucher CM131 chloroplast, complete genome.
Taxon:	Utricularia amethystina
Original size:	150020 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46252	46806	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccccgg aataaaaaaa
101   tgggcaatcc tgagccaaat cctgttttct caaaagaaag gttcataaac
151   agaaaaaaaa ggataggtcc agagactcaa cggaagttgt tctaggaaaa
201   gggggcggaa tctttacacc gaaacgtcat aaagtttcaa ggataaacct
251   acctattgaa tactatatca aacgagtaat catggcctga atctctagat
301   gtattttttt atatgaaaaa tcaaagaatt ggtgtgaatt ccttcgccat
351   tgaagcacaa ctcgaatttt tattcatcaa atcattcgtt cgctccctat
401   ccctagtccg ataaaacttt tcaagaactg atcaattgga caagaataaa
451   gatagagtcc cctccgacat gtcaatgcgg acaacaatga aatttatagt
501   aagaggaaaa tccgttgact tttaaaatca tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details