JRC GMO-Amplicons | EURL GMFF

Record ID:	MN223721.1
Database:	nt	Go to original record
Description:	Utricularia amethystina voucher VM1998 chloroplast, complete genome.
Taxon:	Utricularia amethystina
Original size:	150115 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46426	46971	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccccgg aataaaaaaa
101   taggcaatcc tgagccaaat cctgttttct caaaagaaag gttcataaac
151   agaaaaaaaa aggataggtc cagagactca atggaagttg ttctaggaaa
201   tgggggcgga atctttccac cgaaacgtca gaaaggatca aggataaacc
251   tacctattca atactatatc aaacgagtaa tcatggtctg aatctctaga
301   tgtatttttt tatatgaaaa ataaaagaat tggtgtgaat tcctttgcca
351   ttgaaacaaa actagaattt ttattcatca aatcattcac tccctagtcc
401   gataaacctt ttcaagaact gatcaattgg acaagaataa agatagagtc
451   ccctccgaca tgtcaatgcc gacaacaatg aaatttatag taagaggaaa
501   atccgttgac ttttaaaatc atgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details