JRC GMO-Amplicons | EURL GMFF

Record ID:	MN223720.1
Database:	nt	Go to original record
Description:	Utricularia amethystina voucher VM2032 chloroplast, complete genome.
Taxon:	Utricularia amethystina
Original size:	150332 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46195	46740	+	546 bp

Amplicon:

546 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccccgg aatcaaaaaa
101   tgggcaatcc tgagccaaat cctgttttct caaaagaaag gttcataaac
151   agaaaaaaaa aggataggtc cagagactca atggaagttg ttctaggaaa
201   tgggtgcgga atctttccac cgaaacgtca gaaaggatca aggataaacc
251   tacctattga atactatatc aaacgagtaa tcatggcctg aatctctaga
301   tgtatttttt tatatcaaaa ataaacgaat tggtgtgaat tccttcgcca
351   ttgaaccaaa actagaattt ttatttatca aatcattcac tccctagtcc
401   gataaacctt ttcaagaact gatcaattgg acaagaataa agatagagtc
451   ccctccgaca tgtcaatgcc gacaacaatg aaatttatag taagaggaaa
501   atccgttgac ttttaaaatc atgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	546	527	2.46e-08

Match details