JRC GMO-Amplicons | EURL GMFF

Record ID:	MN383192.1
Database:	nt	Go to original record
Description:	Hemiphragma heterophyllum isolate BDXQ1 chloroplast, complete genome.
Taxon:	Hemiphragma heterophyllum
Original size:	152707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47040	47602	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgatcacttt caaattcaga gaaaccccgg aattaataaa
101   aatggggcaa tcctgagcca aatcctgtct tctcaaaaca aaggttcaga
151   aagcgaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggg tagaggaatc ttttcatcga aacttcagaa
251   aggatgaagg ataaacatat ctattgaata ctatatcaaa taattaatga
301   tgggccaagt ctgtatctgt atttttttag atgaaaaata gaagacttgt
351   tgtgaattga ttccacattc aataaagaat cgaatattca tttatcaaat
401   aacccactcc atagtctgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccgac aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details