JRC GMO-Amplicons | EURL GMFF

Record ID:	EF538134.1
Database:	nt	Go to original record
Description:	Senecio saxatilis tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Senecio saxatilis
Original size:	939 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	530	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcacgtttt ccgaaaacaa acaaagattc
151   agaaagcgaa aatcaaaaag gataggtgca gagactcgat ggaagttgtt
201   ctaacgaatg gagttgattg tcttacattg gtagaggaat ccttctatcg
251   aaacttcaga aaagatgaag gataaacctg tatacataat acagaagaat
301   tggtatgaat cgattccata ttgaaattga agaaagaatc gaatatccat
351   tgatcaaaga tcaaaccatt cactccataa tctgatagat cttttgaaga
401   actgattaat cggatgagaa taaagataga gtcccgttct acatgtcaat
451   accggcaaca atgaaattta tagtaagagg aaaatccgtc gatttcaaaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details