JRC GMO-Amplicons | EURL GMFF

Record ID:	MN309825.1
Database:	nt	Go to original record
Description:	Festuca rubra subsp. rubra chloroplast, complete genome.
Taxon:	Festuca rubra subsp. rubra
Original size:	133804 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45571	46180	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg aggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttccaaa
251   ttttagaaag aagggcttta tacacctaat aaacacgtat agatactgac
301   gattaatcac ggaacccata ttataatata ggttctttat tcttttttag
351   aatgaaattt gaaatagaaa tgattatgaa ataaaaaatt ctgaattttt
401   tgtagaatta tttggaatcc attccaattg aatatttagt aatcaaatcc
451   ttcaattcaa agttttgagg tctttaaaac agtggattaa tcggacgagg
501   acaaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details