JRC GMO-Amplicons | EURL GMFF

Record ID:	MN309822.1
Database:	nt	Go to original record
Description:	Festuca brevipila chloroplast, complete genome.
Taxon:	Festuca brevipila
Original size:	133331 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45348	45953	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg aggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gaattaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttttagaaag aaggtataca cctaataaac acgtatagat actgacgatt
301   aatcatggaa cccatattat aatataggtt ctttattctt ttttagaatg
351   aaatttgaaa tagaaatgat tatgaaataa aaaatttaga attttttgta
401   gaattatttg gattccattc caattgaata tttagtaatc aaatccttca
451   attcaaagtt ttgaggtctt taaaacagtg gattaatcgg acgaggacaa
501   agagagagtc ccattctaca tgtcaatact gacaacaatg aaatttctag
551   taaaaggaaa atccgtcgac tttataagtc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details