JRC GMO-Amplicons | EURL GMFF

Record ID:	MK593558.1
Database:	nt	Go to original record
Description:	Melinis minutiflora voucher K:MSV609 chloroplast, complete genome.
Taxon:	Melinis minutiflora
Original size:	139773 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48703	49320	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattacg ttgtgttggt agtggaactc cctcgaaatt
251   atagaaagaa gggctttata catctaatac acacatagat actgacatag
301   caaacgatta atcacagaac ccatatcata atataggttc cttattttat
351   atattttttt tttagaatga aattaggaat gattatgaaa tagaaaattc
401   tgaatttttt gagaattatt gtgaatccat tccaatcgaa tattgagtaa
451   tcaaatcctt caattcattg ttttgagata tttttaaaaa tggattaatc
501   gaacgaggat aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details