JRC GMO-Amplicons | EURL GMFF

Record ID:	MK593551.1
Database:	nt	Go to original record
Description:	Garnotia stricta var. longiseta voucher K:RS1386 chloroplast, complete genome.
Taxon:	Garnotia stricta var. longiseta
Original size:	139831 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48736	49263	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgagaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaaaaaac aagtggttct
151   caaactagaa cccaaaggaa aaggataggt gcagagactc aatggaagct
201   gttctaacga atcgaagtaa taacgattaa tcacagaacc catattataa
251   tataggttcc ttattttatt ttgagaatga aattaggaat gattatgaaa
301   tagaaaattc tgaatttttt tagaattatt gtgaatctat tccaatcgaa
351   tattgagtaa tcaaatcctt caattcattg ttttgagatc ttttcaaaag
401   tgtattaatc ggacgaggat aaagagagag tcccattcta catgtcaata
451   ctgacaacaa tgaaatttct agtaaaagga aaatccgtcg actttataag
501   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details