JRC GMO-Amplicons | EURL GMFF

Record ID:	MK593549.1
Database:	nt	Go to original record
Description:	Dichanthium aristatum isolate 08Da chloroplast, complete genome.
Taxon:	Dichanthium aristatum
Original size:	138088 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46545	47075	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataattat
251   aatataggtt ctttatttta tttttcgaat gaaattagga atgattatga
301   aatagaaaat tcataatttt ttgagaatta ttgtgaatct attccaatcg
351   aatattgagt aatcaaatcc ttcaattcat tgttttcgag atcttttaaa
401   aagtggatta atcggacgag gataaagaga gagtcccatt ctacatgtca
451   atactgacaa caatgaaatt tctagttaaa ggaaaatccg tcgactttat
501   aagtcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details