JRC GMO-Amplicons | EURL GMFF

Record ID:	MK946456.1
Database:	nt	Go to original record
Description:	Rungia pectinata chloroplast, complete genome.
Taxon:	Rungia pectinata
Original size:	149627 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46587	47138	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aaagggcaat cctgagccaa ctcctatttt cgaaagacaa aggttcagaa
151   aacgaaaaag ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttggct gcattggtag aggaattgtt ccatcaaaac ttccgaaaga
251   ataaacatat ctattgaata ctatattaac agattaacag attagatggc
301   ccacatctgt atttttttta tatggaagaa ttgatgtgaa ttgattccac
351   attgaagaaa gagtccaata ttcattcatc aaatcattca ctccatagtc
401   taatcgttct tttattttaa agaattaatt aagcggacga gaataaagat
451   agagtcccat tccacatgtc aatactggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgactttc aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details