JRC GMO-Amplicons | EURL GMFF

Record ID:	MN380671.1
Database:	nt	Go to original record
Description:	Cornus florida var. urbiniana isolate FCN17100 chloroplast, complete genome.
Taxon:	Cornus florida var. urbiniana
Original size:	157584 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49120	49706	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acgaaggttc
151   ggaaagcgaa aatcaaaaaa gggataggtg cagagactca atggaagctg
201   ttctaacacg tggagttgac taagttgtgt tgttagaaga atcttttttc
251   atcgaaactt cagaaaggat gaaggagaaa actatataca tacgtatacg
301   tactgaaata ttatatcaaa tgattactga cgacacgaat ctttattttt
351   ttatatgaaa aatcaaagaa ttgttgtgaa tcgatttcaa attgaagaaa
401   gaatcaaata ttcattgatc aaatcattca ctccgtagtc tgatatatct
451   tttgaagaac tgattaatcg aacgagaata aagatagagt cccattctac
501   atgtcaatac cgacaacaat gaaatttata gtaagaggaa aatccgtcga
551   ttttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details