JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876652.1
Database:	nt	Go to original record
Description:	Trigonostemon magnificus tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Trigonostemon magnificus
Original size:	977 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gatccaaatc ctgttttcca aaagcaaaca aaggttcaaa
151   aagacagact aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagtgactgc gttgcattag taaagtaaag aaatccttcc
251   cccgaaagga taaagtaaag gataacccta tatacatacg tatacgtatt
301   gaaatactat ctcaaatgat taatcaaatg attaataggg gccgaatcca
351   tattttttta tctttatatg aaatatgaaa aaaaaatgaa ataaaaaaaa
401   attgttttga attgattcca aattgaagaa aggattgaat tttaattgat
451   caaatcattc actccatagt ctgatagata actgattaat cggacgagaa
501   taaagataga gtcccattct acatgtcaat atcgacaaca aggaaactta
551   tagtaagagg aaaatccgtc gacttttaaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details