JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876647.1
Database:	nt	Go to original record
Description:	Trigonostemon hartleyi tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Trigonostemon hartleyi
Original size:	979 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	619	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcca aaagcaaaca aaggttcaaa
151   aagacagaat aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaataaatg tagttgactg cgttgcatta gtaaagtaaa gaaatccttc
251   cgtcaaaact ccagaaagga taaagtaaag gataacccta tatacatacg
301   tatacgtatt gaaatactat ctcaaatgat taatcaaatg attaataggg
351   gccgaatcca tattttttta tctttatatg aaatatgaaa aaaaaatgaa
401   ataaaaaaaa attgttttga attgattcca aattgaagaa aggattgaat
451   taaaattgat caaatcattc actccatagt ctgatagata actgattaat
501   cggacgagaa taaagataga gtcccattct acatgtcaat atcgacaaca
551   aggaaattta tagtaagagg aaaatccgtc nactttaaaa atcgtgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details