JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876644.1
Database:	nt	Go to original record
Description:	Trigonostemon diffusus tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Trigonostemon diffusus
Original size:	836 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	535	+	534 bp

Amplicon:

534 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcca aaaacaaaca aaggttcaaa
151   aagcagaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgcgt tgcattagta aagtaaagaa atccttccgt
251   caaaactcca gaaaggataa agtaaaggat aaccctatat acatangtat
301   acgtattgaa atactatctc aaatgattaa tcaaatgatt aataggggcc
351   gaatccatat ttttttatct ttatatgaaa tatgaaaaaa aaaaaatgaa
401   ataaaaaaaa aggattgaat tttaattgat caaatcattc acgccatagt
451   ctgatagata actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat tgtggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	534	515	2.40e-08

Match details