JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876638.1
Database:	nt	Go to original record
Description:	Trigonostemon lii tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Trigonostemon lii
Original size:	972 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	617	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagccc tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   aggcaatcct gagccaaatc ctgttttcca aaaacaaaca aaggttcaaa
151   aagacagaat aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttgcatta gtaaagtaaa gaaatccttc
251   cgtcaaaact ccagaaagga taaagtaaag gataacccta tatacatacg
301   tatacgtatt gaaatactat ctcaaatgat taatcaaatg attaataggg
351   gccgaatcca tattttttta tctttatatg aaatatgaaa aaaaatgaaa
401   taaaaaaaat tgttttgaat taattccaaa ttgaagaaag gattgaattt
451   taattgatca aatcattcac tccatagtct gatagataac tgattaatcg
501   gacgagaata aagatagagt cccattctac atgtcaatat cgacaacaag
551   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details