JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876620.1
Database:	nt	Go to original record
Description:	Dimorphocalyx moluccensis tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Dimorphocalyx moluccensis
Original size:	961 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	609	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttaattg gattggascc ttggtacgga
51    aacttgctaa gtgataactt tcaaattcag agaaaccctg gaattaaaaa
101   tgggcaatcc tgagccaaat cctgttttcc gaaaacaaac aaaggttcaa
151   aaagacagac taaaaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgaat gcgttgcatt agtaaagtaa araaatcctt
251   ccgtcaaaac tccagaaagg ataaagtaaa ggataaccct atatacatac
301   gtatacgtat tgaaatacta tctcaaatga ttaatcaaat gattaatagg
351   ggccgaatcc atattttttt atctttatat gaaaaatgaa ataaaaaaaa
401   attgttttga attgattcca agttgaagaa aggattgaat tttaattgat
451   caaatcattc actccatagt ctgatagata actgattaat cggacgagaa
501   taaagataga gtcccattct acatgtcaat atcgacaaca aggaaattta
551   tagtaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details