JRC GMO-Amplicons | EURL GMFF

Record ID:	MK876615.1
Database:	nt	Go to original record
Description:	Dimorphocalyx beddomei tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Dimorphocalyx beddomei
Original size:	966 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	611	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtacggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaaa caaaggttca
151   aaaagacaga ctaataaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttgcat tagtaaagta aagaaatcct
251   tccgtcaaaa ctccagaaag gataaagtaa aggataaccc tatatacata
301   cgtatacgta ttgaaatact atctcaaatg attaatcaaa tgattaatag
351   gggccgaatc catatttttt tatctttata tgaaaaatga aataaaaaaa
401   aattgttttg aattgattcc aagttgaaga aaggattgaa ttttaattga
451   tcaaatcatt cactccatag tctgatagat aactgattaa tcggacgaga
501   ataaagatag agtcccattc tacatgtcaa tatcgacaac aaggaaattt
551   atagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details