JRC GMO-Amplicons | EURL GMFF

Record ID:	MK358447.1
Database:	nt	Go to original record
Description:	Impatiens glandulifera isolate HB92 chloroplast, complete genome.
Taxon:	Impatiens glandulifera
Original size:	152260 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46657	47252	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tggaaaacaa atctaaataa
151   agattccgaa accaaaaata gaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tagtagagaa atccgtggat
251   cgaaacttca gaaagtatga aagaaaaaca tatatacata tgcatatatg
301   ctgaaatact atatcaatgg tatatcaaaa taaaatgaat gacgatcaaa
351   aagcgtatcc cttttttcct aaaaaaaagg gttgtgaatc gattccatat
401   ttgaataaag aatcaaatat tcattggtca aatcatttac tccaaagtgt
451   gatagttcct ctgaagaact tattaatcag acgagaataa agatagagtc
501   ccattataca tgtcaatccc gacaacaatg aaatttatag taataggaaa
551   atccgtcgac tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details