JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395451.1
Database:	nt	Go to original record
Description:	Tsuga sieboldii voucher YU Havill 05-01 tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tsuga sieboldii
Original size:	1448 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	470	1025	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccccggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatttca tttggggtcc aatactctat ttatagaacg ctttatttac
251   acctcgaaag tgggaatgtt gtataaaata aaacaacact cgcgatcaga
301   acttgaatcg tttcaagcat tttttttgta agagaaatgc cagatttgag
351   ttgaagtaat gatttgacat taagtaatcc aattatgaac tttctctact
401   ctagataggg agttgaatca gtttttggaa gaaattattg gacgaggata
451   aagatagagt ccaattctac gtgtcaatgt caacaacaat gcaaattgca
501   gtaggaggaa aatccgttgg cttatagacc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details