JRC GMO-Amplicons | EURL GMFF

Record ID:	EF395446.1
Database:	nt	Go to original record
Description:	Tsuga mertensiana voucher YU Havill 03-03a tRNA-Leu (trnL) gene, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tsuga mertensiana
Original size:	1458 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	475	1030	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttgatcg aattgagcct tggtatggaa
51    acctaccaag tgatagcttc caaattcagg gaaccccggg atattttgaa
101   tgggtaatcc tgagccaaat ccggttcata gagacaatgg tttctttcct
151   aggataggaa ggggataggt gcagagactc aatggaagct attctaacga
201   atgaatttca tttggggtcc aatactctat ttatagaacg ctttatttac
251   acctcgaaag tgggaatgtt gtataaaatc aaacaacact cgcgatcaga
301   actggaatcg ttccaagcat tttatttgta agagaaatgc cagatttgag
351   ttgaagtaat gatttgacat taagtaatcc aattatgaac ttctctactc
401   tagataggga gttgaatcag tttttggaat aaatgattgg acgaggataa
451   agatagagtc caattctacg tgtcaatgtc aacaacaatg caaattgcag
501   taggaggaaa atccgttggc tttatagacc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details